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M94A3187.TXT
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1994-10-25
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Document 3187
DOCN M94A3187
TI Evaluation of immunoassays for detection of HTLV-I/II antibodies.
DT 9412
AU Andersson S; Godoy K; Thorstensson R; Dias F; Biberfeld G; Dept. of
Immunology, Swedish Institute for Infectious Disease; Control,
Stockholm, Sweden.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):133 (abstract no. PA0151). Unique
Identifier : AIDSLINE ICA10/94369388
AB BACKGROUND: A general screening of blood donors for HTLV-I/II infection
during one year from the beginning of 1994 has been initiated in Sweden.
OBJECTIVE: To assess the sensitivity and specificity of commercially
available screening assays for HTLV-I/II antibodies. METHODS: Serum
panels (Swedish) 500-628 unselected fresh blood donor sera, 400
unselected frozen blood donor sera, 12 selected HTLV-II positive sera
from IVDUs. West African (Guinea-Bissau) 400 unselected frozen sera from
medical and surgical in-patients at the National Hospital, Bissau (347
negative, 22 HTLV-I positive and 31 ID (excluded)), 79 selected HTLV-I
positive sera and 6 selected HTLV-II positive sera. The performance of 5
different ELISA tests (see below) were evaluated. RESULTS: The
sensitivity for HTLV-I was 100% for all assays. The Murex/Cambridge EIA
detected all 18 HTLV-II samples. The other 4 assays missed one HTLV-II
positive sample each after repeated testing (initially 1-4 false
negative results). Organon missed one Swedish HTLV-II positive sample,
all other false negative HTLV-II positive samples were from Africa.
TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSION: The sensitivity for
HTLV-I and the specificity was high for all assays. The results indicate
that HTLV-II seropositive sera may give false negative results on some
assays. Further characterization of the HTLV-II seropositive samples
with PCR and virus isolation is under way and may give more light to the
results obtained. Evaluations of other assays for detection of
antibodies to HTLV are ongoing and the results will be presented.
DE Blood Donors Blood Specimen Collection Comparative Study
Enzyme-Linked Immunosorbent Assay/*METHODS False Negative Reactions
Guinea-Bissau Human HTLV-I Antibodies/*BLOOD HTLV-II
Antibodies/*BLOOD HTLV-II Infections/BLOOD/*DIAGNOSIS/IMMUNOLOGY
Inpatients Polymerase Chain Reaction/METHODS Sensitivity and
Specificity Sweden MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).